EEF2K (phospho-S366) polyclonal antibody
Product Specifications
Background
EEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K
Swiss Prot
O00418
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human eEF2K around the phosphorylation site of Serine 366.
Conjugation
Unconjugated
Related Pathways
Signaling Pathways Activating p38 MAP Kinase
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 105 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-eEF2K (S366) polyclonal antibody detects endogenous levels of eEF2K protein when phosphorylated at Ser366.
Applications Notes
Western blot (WB) analysis of p-eEF2K (S366) pAb at 1:1000 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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