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Bcl-X (phospho-S62) polyclonal antibody

Product Specifications

Background

Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Bcl-x, one of several additional proteins with sequence homology to Bcl-2, is expressed as Bcl-xL, a 233 amino acid protein with 43% sequence identity with Bcl-2 that suppresses cell death, and Bcl-xS, a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188) found in Bcl-xL and which functions as a dominant inhibitor of Bcl-2. A further apoptosis-inducing protein, Bad, dimerizes both with Bcl-xL and to a lesser extent with Bcl-2, thus displacing Bax and inducing apoptosis

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCL2L; BCLX; Bcl-xL; Bcl-XS; BclxL; BclXS

Swiss Prot

Q07817

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Bcl-X around the phosphorylation site of Serine 62.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:150

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 26 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Bcl-X (S62) polyclonal antibody detects endogenous levels of Bcl-X protein only when phosphorylated at Ser62.

Applications Notes

Western blot (WB) analysis of Bcl-X (phospho-S62) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug)

Host or Source

Rabbit

Available Sizes

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