Bcl-2 (phospho-S70) polyclonal antibody
Product Specifications
Background
Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Apoptosis regulator Bcl-2; Bcl 2; BCL2
Swiss Prot
P10415
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Serine 70.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 33 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Bcl-2 (S70) polyclonal antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at Ser70.
Applications Notes
Western blot (WB) analysis of Bcl-2 (phospho-S70) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with UV for 1h whole cell lysate (40ug) Lane3:HEK293T treated with UV for 6h whole cell lysate (40ug) Lane4:HEK293T treated with UV for 24h whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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