AMPKα1 (phospho-S486) polyclonal antibody

AMPK is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; au-protein kinase PRKAA1; PRKAA1; AMPK1

Hypoxia Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Insulin &Glucose Signaling, AMPK Signaling Pathway, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease, MTOR signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

P-AMPKα1 (S486) polyclonal antibody detects endogenous levels of AMPKα1 protein only when phosphorylated at Ser486. The antibody does not cross-react with phosphorylated AMPKα2 or other related proteins.

Western blot (WB) analysis of p-AMPKα1 (S486) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with UV Lane2:sp2/0 cell lysate treated with UV Lane3:PC12 cell lysate treated with UV