14-3-3 ζ (phospho-S58) polyclonal antibody

Product Specifications

Background

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; 14-3-3 protein zeta; 14-3-3 protein delta; 14-3-3 ζ; 14-3-3 δ; 1433Z; KCIP1; KCIP 1

Swiss Prot

P63104

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Histone 14-3-3 ζ around the phosphorylation site of Serine 58.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 28 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-14-3-3 ζ (S58) polyclonal antibody detects endogenous levels of 14-3-3 protein zeta/delta only when phosphorylated at Ser58.

Applications Notes

Western blot (WB) analysis of 14-3-3 ζ (phospho-S58) polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate (40ug) Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)