Rab 6A (K144) polyclonal antibody

Product Specifications

Background

Rab proteins are also an integral part of endocytic pathways. Rab 6A is a ubiquitously expressed member of the Rab family of proteins and localizes to the Golgi membrane where it regulates retrograde transport from the late endosomes via the Golgi to endoplasmic reticulum (ER) pathway. Three isoforms exist due to alternative splicing events, namely the ubiquitously expressed isoforms Rab 6A' and Rab 6A, and the brain-specific isoform Rab 6B.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ras-related protein Rab-6A; Rab-6; RAB6A; RAB6

Swiss Prot

P20340

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-170 of Human Rab 6A.

Conjugation

Unconjugated

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 24 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Rab 6A (K144) polyclonal antibody detects endogenous levels of Rab 6A protein.

Applications Notes

Western blot (WB) analysis of Rab 6A (K144) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:The Testis tissue lysate of Rat (40ug)