Glut 4 (R271) polyclonal antibody
Product Specifications
Background
In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Solute carrier family 2; facilitated glucose transporter member 4; Glucose transporter type 4; insulin-responsive; GLUT-4; GLUT4; SLC2A4
Swiss Prot
P14672
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 250-300 of Human Glut 4.
Conjugation
Unconjugated
Related Pathways
Warburg Effect, Insulin &Glucose Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 45-55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Glut4 (R271) polyclonal antibody detects endogenous levels of Glut4 protein.
Applications Notes
Western blot (WB) analysis of Glut 4 (R271) pAb at 1:500 dilution Lane1:The Heart tissue lysate of Mouse (40ug) Lane2:The Heart tissue lysate of Rat (20ug) Lane3:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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