Cardiotrophin-1 (H31) polyclonal antibody

Product Specifications

Background

Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and has been shown to induce hypertrophy in cardiac myocytes in vitro. Cardiotrophin-1, a secreted protein expressed at high levels in myocardium during cardiogenesis, has been shown to promote proliferation and survival of embryonic cardiomyocytes, suggesting a role for Cardiotrophin-1 in the activation of gp130 during cardiac development.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cardiotrophin-1; CT-1; CTF1; Cardiotrophin1; CT1; Cardiotrophin 1; CT 1

Swiss Prot

Q16619

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 1-53 of Human CT-1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 27 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cardiotrophin-1 (H31) polyclonal antibody detects endogenous levels of Cardiotrophin-1 protein.

Applications Notes

Western blot (WB) analysis of Cardiotrophin-1 (H31) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:K562 whole cell lysate (20ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:The Muscle tissue lysate of Mouse (40ug) Lane5:H9C2 whole cell lysate (40ug)