VASP (A232) polyclonal antibody
Product Specifications
Background
VASP (vasodilator-stimulated phosphoprotein), is involved in the maintenance of cytoarchitecture by interacting with actin-like filaments. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in WAS patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is a protein of between 46 and 50 kDa that is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Vasodilator-stimulated phosphoprotein; VASP
Swiss Prot
P50552
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 200-250 of Human VASP.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 46, 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
VASP (A232) polyclonal antibody detects endogenous levels of VASP protein.
Applications Notes
Western blot (WB) analysis of VASP (A232) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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