CD63 (K156) polyclonal antibody

Product Specifications

Background

CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD63; MLA1; TSPAN30

Swiss Prot

P08962

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 111-160 of Human CD63.

Conjugation

Unconjugated

Related Pathways

Tumor-Infiltrating Immune Cell Marker Guide, Contribution of ECM and Cytoskeletal Factors to EMT

Applications

WB IHC

Dilution

WB: 1:500~1:2000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 25-60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD63 (K156) polyclonal antibody detects endogenous levels of CD63 protein.

Applications Notes

Western blot (WB) analysis of CD63 (K156) pAb at 1:1000 dilution Lane1:C6 whole cell lysate (30ug) Lane2:SP2/0 whole cell lysate (30ug) Lane3:K562 whole cell lysate (30ug) Lane4:A549 whole cell lysate (30ug)