NOC3L (F447) polyclonal antibody

Product Specifications

Background

GADD 153, a growth arrest and DNA damage-inducible gene, encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10 or C/EBP zeta) . GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase during cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from “classical” C/EBP binding sites, recognizing instead unique “nonclassical” sites. Thus, GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells. GADD 153 belongs to the CBF/MAK21 family, which also includes NOC2L, NOC3L and NOC4L. NOC3L, also designated factor for adipocyte differentiation 24 or Fad24, promotes adipogenesis by controlling DNA replication during the early stages of mitotic clonal expansion (MCE) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nucleolar complex protein 3 homolog; NOC3 protein homolog; Factor for adipocyte differentiation 24; NOC3-like protein; Nucleolar complex-associated protein 3-like protein; NOC3L; AD24; C10orf117; FAD24

Swiss Prot

Q8WTT2

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 417-436 of Human NOC3L.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 110 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NOC3L (F447) polyclonal antibody detects endogenous levels of NOC3L protein.

Applications Notes

Western blot (WB) analysis of NOC3L (F447) pAb at 1:1000 dilution Lane1:HCT116 whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (20ug) Lane3:AML-12 whole cell lysate (20ug) Lane4:H9C2 whole cell lysate (40ug)