ZBP-89 (E84) polyclonal antibody

Product Specifications

Background

ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1) . ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Zinc finger protein 148; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; ZNF148; ZBP89

Swiss Prot

Q9UQR1

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 53-98 of Human ZBP-89.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 100-120 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ZBP-89 (E84) polyclonal antibody detects endogenous levels of ZBP-89 protein.

Applications Notes

Western blot (WB) analysis of ZBP-89 (E84) at 1:500 dilution Lane1:H9C2 whole cell lysate (40ug) Lane2:SP2/0 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug)