COL4A2 (P175) polyclonal antibody
Product Specifications
Background
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (Types I, II, III and V) and basement membrane collagens (Type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Collagen alpha-2 (IV) chain; Canstatin; COL4A2
Swiss Prot
P08572
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 131-180 of Human COL4A2.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 180 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
COL4A2 (P175) polyclonal antibody detects endogenous levels of COL4A2 protein.
Applications Notes
Western blot (WB) analysis of COL4A2 (P175) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:Panc1 whole cell lysate (40ug) Lane5:H9C2 whole cell lysate (40ug) Lane6:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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