TIS11D (H243) polyclonal antibody

Product Specifications

Background

TIS11D, also known as ZFP36LA (zinc finger protein 36, C3H type-like 2), BRF2 (butyrate response factor 2), ERF2 or RNF162C, is a 494 amino acid protein that localizes to the nucleus and contains two CSH1-type zinc fingers. Belonging to the TIS11 family of early response proteins, TIS11D is thought to function as a nuclear transcription factor that binds to 5'UUAUUUAUUU-3' core RNA sequences and may regulate growth factor-induced cellular responses. The gene encoding TIS11D maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Zinc finger protein 36, C3H1 type-like 2; ZFP36-like 2; Butyrate response factor 2; EGF-response factor 2; ERF-2; Protein TIS11D; ZFP36L2; BRF2; ERF2; RNF162C; TIS11D

Swiss Prot

P47974

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 200-250 of Human TIS11D.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 57 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TIS11D (H243) polyclonal antibody detects endogenous levels of TIS11D protein.

Applications Notes

Western blot (WB) analysis of TIS11D (H243) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:A375 whole cell lysate (40ug) Lane5:Myla2059 whole cell lysate (40ug)