PKA Iβ reg (F138) polyclonal antibody

Product Specifications

Background

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA) . Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CAMP-dependent protein kinase type I-beta regulatory subunit; PRKAR1B

Swiss Prot

P31321

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-160 of Human PKA Iβ reg.

Conjugation

Unconjugated

Related Pathways

Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PKA Iβ reg (F138) polyclonal antibody detects endogenous levels of PKA Iβ reg protein.

Applications Notes

Western blot (WB) analysis of PKA Iβ reg (F138) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse (10ug) Lane2:The Brain tissue lysate of Rat (20ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:K562 whole cell lysate (40ug)