HBO1 (S167) polyclonal antibody

Product Specifications

Background

KAT7 / Hbo1 is a member of the MYST histone acetyltransferae family which acetylates both histone H3 and H4 and is involved in the regulation of replication. The initiation of DNA replication is tightly regulated in eukaryotic cells to ensure that the genome is precisely duplicated once per cell cycle. This is accomplished by controlling the pre-replicative complex. KAT7 acts as a positive regulatory factor for pre-RC assembly and thus controls DNA replication.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone acetyltransferase MYST2; MYST protein 2; MOZ; YBF2/SAS3; SAS2 and TIP60 protein 2; Histone acetyltransferase binding to ORC1; MYST2; HBO1; HBOa

Swiss Prot

O95251

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 141-190 of Human HBO1.

Conjugation

Unconjugated

Related Pathways

Epigenetic Writers and Erasers of Histone H3, Epigenetic Writers and Erasers of Histones H2A H2B and H4

Applications

WB IF IP

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200 IP: 1:10~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HBO1 (S167) polyclonal antibody detects endogenous levels of HBO1 protein.

Applications Notes

Western blot (WB) analysis of HBO1 (S167) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)