BUB1B (T368) polyclonal antibody
Product Specifications
Background
Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L Protein SSK1; BUB1B; BUBR1; MAD3L; SSK1
Swiss Prot
O60566
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 341-390 of Human BUB1B.
Conjugation
Unconjugated
Applications
WB IP
Dilution
WB: 1:500~1:1000 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 130 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
BUB1B (T368) polyclonal antibody detects endogenous levels of BUB1B protein.
Applications Notes
Western blot (WB) analysis of BUB1B (T368) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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