Cdc37 (F259) polyclonal antibody
Product Specifications
Background
Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation..
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Hsp90 co-chaperone Cdc37; Hsp90 chaperone protein kinase-targeting subunit; p50Cdc37; CDC37; CDC37A
Swiss Prot
Q16543
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 226-276 of Human Cdc37.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cdc37 (F259) polyclonal antibody detects endogenous levels of Cdc37 protein.
Applications Notes
Western blot (WB) analysis of Cdc37 (F259) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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