14-3-3 θ (Q67) polyclonal antibody
Product Specifications
Background
Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27 (Kip1) . Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAQ; 14-3-3 θ; 14-3-3 τ; 1433T
Swiss Prot
P27348
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 30-80 of Human 14-3-3 θ.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
14-3-3 θ (Q67) polyclonal antibody detects endogenous levels of 14-3-3 protein theta/tau.
Applications Notes
Western blot (WB) analysis of 14-3-3 θ (Q67) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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