HPK1 (L397) polyclonal antibody

Product Specifications

Background

Several mammalian kinases have been identified with sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include HPK1, KHS, GLK, NIK, YSK1, Krs-1, Krs-2 and GC kinase. HPK1 (hematopoietic progenitor kinase 1), like many other STE20-like kinases, specifically activates the JNK signaling pathway. HPK1 binds to and phosphorylates MEKK, suggesting it plays an important role in regulating the stress responsive JNK/SAPK signaling pathway.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mitogen-activated protein kinase kinase kinase kinase 1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; MAP4K1; HPK1

Swiss Prot

Q92918

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 365-412 of Human HPK1.

Conjugation

Unconjugated

Related Pathways

SAPK JNK Signaling Cascades

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 97 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HPK1 (L397) polyclonal antibody detects endogenous levels of HPK1 protein.

Applications Notes

Western blot (WB) analysis of HPK1 (L397) pAb at 1:500 dilution Lane1:HCC827 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:The Spleen tissue lysate of Mouse (40ug)