TLE4 (H190) polyclonal antibody
Product Specifications
Background
TLEs associate with chromatin in live cells and specifically with Histone H3, but not with other core histones. Expression of the TLE genes, TLE1, TLE2, TLE3 and TLE4, correlate with immature epithelial cells that are progressing toward a terminally differentiated state, suggesting a role during epithelial differentiation. TLE1, TLE2 and TLE3 have elevated expression in cervial squamous metaplasias and carcinomas, while TLE4 is most highly expressed in the brain, particularly in the caudate nucleus. TLE1 and TLE4 contain SP and WD40 domains, through which TLE1 binds AML1 to inhibit AML1-induced transactivation of the CSF1 receptor. In early stages of cell differentiation, TLE1 is up-regulated, and TLE2 and TLE4 are down-regulated.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Transducin-like enhancer protein 4; TLE-4; KIAA1261
Swiss Prot
Q04727
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 170-230 of Human TLE4.
Conjugation
Unconjugated
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 83 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TLE4 (H190) polyclonal antibody detects endogenous levels of TLE4 protein.
Applications Notes
Western blot (WB) analysis of TLE4 (H190) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (10ug) Lane3:The Testis tissue lysate of Mouse (40ug) Lane4:The Testis tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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