Sp3/4 (G659) polyclonal antibody
Product Specifications
Background
The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1) . Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the β4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Transcription factor Sp3; SPR-2; SPR2; SP3; Transcription factor Sp4; SPR-1; SPR1; SP4
Swiss Prot
Q02447/Q02446
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 630-680 of Human Sp3.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 85 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Sp3/4 (G659) polyclonal antibody detects endogenous levels of Sp3 and Sp4 protein.
Applications Notes
Western blot (WB) analysis of Sp3/4 (G659) pAb at 1:1000 dilution Lane1:L02 whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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