Cyclophilin F (Y121) polyclonal antibody

Product Specifications

Background

Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Cyclophilin F (CyP3), is also known as PPIF, Cyp-D or Rotamase F, is a 207 amino acid mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F; PPIF; CYP3

Swiss Prot

P30405

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 91-140 of Human Cyclophilin F.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cyclophilin F (Y121) polyclonal antibody detects endogenous levels of Cyclophilin F protein.

Applications Notes

Western blot (WB) analysis of Cyclophilin F (Y121) pAb at 1:1000 dilution Lane1:The Kidney tissue lysate of Mouse (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug)