UMP-CMP Kinase (E36) polyclonal antibody

Product Specifications

Background

Nucleoside monophosphate kinases are required for pharmacological activation of therapeutic nucleosides and nucleotide analogs. CMPK (Cytidine monophosphate kinase), also known as UMP-CMP kinase and Deoxycytidylate kinase, is a 196 amino acid protein that catalyzes the phosphoryl transfer from ATP to UMP, CMP and dCMP. This enzymatic reaction leads to the formation of ADP and the corresponding nucleoside diphosphate, which are required for cellular nucleic acid synthesis. Primarily localized to the cytoplasm, CMPK also plays an important role in the activation of pyrimidine analogs, which is clinically useful anti-cancer and anti-viral drugs. CMP is the best substrate for CMPK, followed by UMP and dCMP.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

UMP-CMP kinase; Deoxycytidylate kinase; dCMP kinase; Nucleoside-diphosphate kinase; Uridine monophosphate/cytidine monophosphate kinase; UMP/CMP kinase; UMP/CMPK; CMPK1; CMK; CMPK; UCK; UMK; UMPK

Swiss Prot

P30085

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminal of Human UMP-CMP Kinase.

Conjugation

Unconjugated

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 26 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

UMP-CMP Kinase (E36) polyclonal antibody detects endogenous levels of UMP-CMP Kinase protein.

Applications Notes

Western blot (WB) analysis of UMP-CMP Kinase (E36) pAb at 1:1000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:MEF whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)