TCF-4/12 (S616) polyclonal antibody
Product Specifications
Background
T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to β-catenin in a cooperative manner. This TCF-4/β-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2; TCF4; BHLHB19; ITF2; SEF2; Transcription factor 12; TCF-12; Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4; TCF12; BHLHB20; HEB; HTF4
Swiss Prot
P15884/Q99081
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 580-630 of Human TCF-4.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 60, 72 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TCF-4/12 (S616) polyclonal antibody detects endogenous levels of TCF-4, and also detects TCF-12 protein.
Applications Notes
Western blot (WB) analysis of TCF-4/12 (S616) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug) Lane6:AML-12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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