14-3-3 ζ (W228) polyclonal antibody

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; 14-3-3 protein zeta; 14-3-3 protein delta; 14-3-3 ζ; 14-3-3 δ; 1433Z; KCIP1; KCIP 1

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of 14-3-3 ζ (W228) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:A549 whole cell lysate (20ug) Lane3:The Lung tissue lysate of Rat (40ug) Lane4:AML-12 whole cell lysate (40ug)