AhR (K32) polyclonal antibody

Product Specifications

Background

The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) .Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt) . The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR; BHLHE76

Swiss Prot

P35869

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids N-terminus of Human AhR.

Conjugation

Unconjugated

Applications

WB IHC IF IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 94 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AhR (K32) polyclonal antibody detects endogenous levels of AhR protein.

Applications Notes

Western blot (WB) analysis of AhR (K32) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug)