What is the catalog number for PKC-pan (T495) polyclonal antibody?

The catalog number for PKC-pan (T495) polyclonal antibody is BS1580.

What sizes are available for PKC-pan (T495) polyclonal antibody?

PKC-pan (T495) polyclonal antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does PKC-pan (T495) polyclonal antibody come in?

PKC-pan (T495) polyclonal antibody is available in 2 different pack sizes.

Is PKC-pan (T495) polyclonal antibody in stock?

PKC-pan (T495) polyclonal antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

PKC-pan (T495) polyclonal antibody

Product Specifications

Background

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ) . Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA; Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB; Protein kinase C gamma type; PKC-gamma; PKCG; PRKCG; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Sphingosine-dependent protein kinase-1; SDK1; PRKCD; Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE; Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; PKC zeta; Protein kinase C theta type; nPKC-theta; PRKCQ; PRKCT

Swiss Prot

P17252/P05771/Q05655/Q02156/P05129/P24723/Q04759/Q05513

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 470-520 of Human PKC-pan.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, Phospholipase Signaling, Protein Kinase C Signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis

Applications

WB IHC IF IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~75 to 88 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PKC-pan (T495) polyclonal antibody detects endogenous levels of PKC α, β I, β II, γ, δ, ε, η, ζ and θ isoforms.

Applications Notes

Western blot (WB) analysis of PKC-pan (T495) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:COS-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug)