PKC ζ (P556) polyclonal antibody
Product Specifications
Background
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, Βii and γ) and novel (n) PKC isoforms (δ, ε , ζ, η and θ) . Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca++. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; PKC zeta
Swiss Prot
Q05513
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 520-570 of Human PKC ζ.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, Protein Kinase C Signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Mitochondrial Control of Apoptosis
Applications
WB IHC IP IF
Dilution
WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 72 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PKC ζ (P556) polyclonal antibody detects endogenous levels of PKC ζ protein.
Applications Notes
Western blot (WB) analysis of PKC ζ (P556) polyclonal antibody at 1:2000 dilution Lane1:The Testis tissue lysate of Mouse (40ug) Lane2:PC12 cell lysate Lane3:A549 cell lysate Lane4:U-87MG cell lysate
Host or Source
Rabbit
Available Sizes
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