PIAS3 (F25) polyclonal antibody

Product Specifications

Background

The IL-6 type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization, and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

E3 SUMO-protein ligase PIAS1; Protein inhibitor of activated STAT protein 1; Gu binding protein; GBP; RNA helicase II binding protein; DEAD/H box-binding protein 1; DDXBP1

Swiss Prot

Q9Y6X2

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human PIAS3.

Conjugation

Unconjugated

Related Pathways

Jak Stat : IL-6 Receptor Signaling

Applications

WB IF

Dilution

WB: 1:5000~1:10000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 75 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PIAS3 (F25) polyclonal antibody detects endogenous levels of PIAS3 protein.

Applications Notes

Western blot (WB) analysis of PIAS3 (F25) polyclonal antibody at 1:5000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:HeK293T whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:MEF whole cell lysate (40ug) Lane6:The Testis tissue lysate of Mouse (40ug) Lane7:The Brain tissue lysate of Rat (40ug)