Smad2 (Ab-467) polyclonal antibody

Product Specifications

Background

Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7. Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2

Swiss Prot

Q15796

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human Smad2.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Contribution of Soluble Factors to EMT, hippo Signaling, TGFβ Smad signaling

Applications

WB IP

Dilution

WB: 1:500~1:1000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Smad2 (Ab-467) polyclonal antibody detects endogenous levels of Smad2 protein.

Applications Notes

Western blot (WB) analysis of Smad2 (Ab-467) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (30ug) Lane2:Hela whole cell lysate (30ug) Lane3:PC12 whole cell lysate (30ug) Lane4:3T3-L1 whole cell lysate (30ug)