Caspase-8 (S347) polyclonal antibody
Product Specifications
Background
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition) . Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Caspase-8; Caspase8; Caspase 8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; Caspase-8 subunit p18; Caspase-8 subunit p10; CASP8; MCH5
Swiss Prot
Q14790
Reactivity
Human, Rat
Immunogen
Synthetic peptide, corresponding to Human Caspase-8.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Necrotic Cell Death, Inhibition of Apoptosis, Toll-like Receptor signaling, Death Receptor Signaling
Applications
WB IHC IP
Dilution
WB: 1:500~1:2000 IHC: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Caspase 8 (S347) polyclonal antibody detects endogenous levels of Caspase 8 protein.
Applications Notes
Western blot (WB) analysis of Caspase-8 pAb at 1:2000 dilution Lane1:PC12 whole cell lysate (30ug) Lane2:BV2 whole cell lysate (30ug) Lane3:A549 whole cell lysate (30ug) Lane4:MCF-7 whole cell lysate (20ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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