PSCA (V71) polyclonal antibody

Product Specifications

Background

Prostate stem cell antigen (PSCA) is a 123 amino acid glycosylated protein that shares homology with the Thy-1/Ly-6 family of glycosyl-phosphatidylinositol GPI) -anchored cell surface antigens. The PSCA gene maps to chromosome 8q24.2 and transcripts are most prevalent in prostate and placenta. The gene encoding c-myc is also located on chromosome 8q and like PSCA, is overexpressed in a large number of prostate cancers. Transcripts for PSCA are also abundant in urothelial tumors, and levels of PSCA transcripts increase in confluent RT112 bladder carcinomas, suggesting that PSCA is a marker for urothelial and gastric tissue carcinogenesis. Among prostate cancer cell surface antigens, PSCA is expressed in over 80% of prostate carcinomas and correlates well to certain prostate cancer phenotypes such as prostate cancer bone metastates.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

PSCA; PRO232; Prostate stem cell antigen; UNQ206

Swiss Prot

O43653

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 41-90 of Human PSCA.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 23 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PSCA (V71) polyclonal antibody detects endogenous levels of Prostate Stem Cell Antigen protein.

Applications Notes

Western blot (WB) analysis of PSCA (V71) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)