MCM2 / BM28 (A11) polyclonal antibody
Product Specifications
Background
The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATPbinding motif, which is predicted to mediate ATP-dependent opening of doublestranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the ostreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog Nuclear protein BM28; MCM2; BM28; CCNL1; CDCL1; KIAA0030
Swiss Prot
P49736
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human MCM2.
Conjugation
Unconjugated
Applications
WB IHC IF IP
Dilution
WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 125 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MCM2 (A11) polyclonal antibody detects endogenous levels of MCM2 protein.
Applications Notes
Western blot (WB) analysis of BM28 / MCM2 (A11) pAb at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:The Testis tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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