HDAC1 (E468) polyclonal antibody

Product Specifications

Background

Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone deacetylase 1; HD1; RPD3L1

Swiss Prot

Q13547

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 435-486 of Human HDAC1.

Conjugation

Unconjugated

Related Pathways

Notch Signaling, Nuclear Receptor Signaling, Cell Cycle Control G1 S Checkpoint, DNA Methylation, Epigenetic Writers and Erasers of Histone H3, Epigenetic Writers and Erasers of Histones H2A H2B and H4

Applications

WB IHC IF IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HDAC1 (E468) polyclonal antibody detects endogenous levels of HDAC1 protein.

Applications Notes

Western blot (WB) analysis of HDAC1 (E468) polyclonal antibody at 1:500 dillution Lane1:HEK293T whole cell lysate (40μg) Lane2:Hela whole cell lysate (40μg) Lane3:The Kidney tissue lysate of Mouse (40μg) Lane4:The Kidney tissue lysate of Rat (40μg) Lane5:The Heart tissue lysate of Mouse (40μg) Lane6:The Heart tissue lysate of Rat (40μg)