TFDP1 (V393) polyclonal antibody

Product Specifications

Background

The human retinoblastoma gene product appears to play an important role in the negative egulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 forms heterodimers with a second protein, designated DP-1, forming an “active” E2F transcriptional regulatory complex. Additional members of the E2F family include E2F-2, E2F-3, E2F-4, E2F-5 and DP-2.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transcription factor Dp-1; DRTF1-polypeptide 1; DRTF1; E2F dimerization partner 1; TFDP1; DP1

Swiss Prot

Q14186

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

Conjugation

Unconjugated

Related Pathways

Cell Cycle Control G1 S Checkpoint

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 43 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TFDP1 (V393) polyclonal antibody detects endogenous levels of TFDP1 protein.

Applications Notes

Western blot (WB) analysis of TFDP1 (V393) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:The Muscle tissue lysate of Mouse (40ug)