PTGS1 (S585) polyclonal antibody

Product Specifications

Background

Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid. While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) . PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression. Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock. Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclooxygenase-1; COX-1; Prostaglandin-endoperoxide synthase 1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; PTGS1; COX1

Swiss Prot

P23219

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 555-600 of Human PTGS1.

Conjugation

Unconjugated

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PTGS1 (S585) polyclonal antibody detects endogenous levels of PTGS1 protein.

Applications Notes

Western blot (WB) analysis of PTGS1 (S585) polyclonal antibody at 1:1000 dilution Lane1:CT-26 whole cell lysate (20ug) Lane2:SGC7901 whole cell lysate (20ug)