EIF3H polyclonal antibody

Product Specifications

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3 complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

EIF3H; EIF3S3; eIF3-gamma; eIF3-p40

Swiss Prot

O15372

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human EIF3H (NP_003747.1) .

Conjugation

Unconjugated

Applications

WB IHC IP IF

Dilution

WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:200|IF,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

40kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EIF3H polyclonal antibody detects endogenous levels of EIF3H protein.

Applications Notes

Western blot (WB) analysis of EIF3H pAb at 1:5000 dilution Lane1:EC9706 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:The Pancreas tissue lysate of Mouse (40ug) Lane5:The Thymus tissue lysate of Rat (40ug) Lane6:PC12 whole cell lysate (40ug) Lane7:RAW264.7 whole cell lysate (40ug)