MK2 polyclonal antibody
Product Specifications
Background
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2
Swiss Prot
P49137
Reactivity
Human, Rat, Mouse
Immunogen
Recombinant protein of human MK2.
Conjugation
Unconjugated
Related Pathways
Regulation of Microtubule Dynamics, Signaling Pathways Activating p38 MAP KinaseMAPK Erk in Growth and Differentiation Pathway
Applications
WB IF
Dilution
WB: 1:500~1:1000 IF: 1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 47, 49 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MK2 polyclonal antibody detects endogenous levels of MK2 protein.
Applications Notes
Western blot (WB) analysis of MK2 polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (30ug) Lane2:SGC7901 whole cell lysate (30ug) Lane3:The Brain tissue lysate of Mouse (30ug) Lane4:The Brain tissue lysate of Rat (30ug)
Host or Source
Rabbit
Available Sizes
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