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ENO2 polyclonal antibody

Product Specifications

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ENO2; HEL-S-279; NSE; enolase 2

Swiss Prot

P09104

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ENO2.

Conjugation

Unconjugated

Applications

WB, IF/ICC, IHC, FC

Dilution

WB,1:500 - 1:1000|IF/ICC,1:100 - 1:500|IHC,1:100 - 1:200|FC,1:100 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

48KDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ENO2 polyclonal antibody detects endogenous levels of ENO2 protein.

Applications Notes

Western blot analysis of ENO2 expression in HEK293T (A), Hela (B), U2OS (C), mouse brain (D), mouse eyes (E), rat brain (F), rat liver (G) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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