LC3B polyclonal antibody
Product Specifications
Background
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B
Swiss Prot
Q9GZQ8
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human LC3B (NP_073729.1) .
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB,1:500 - 1:2000|IHC,1:200 - 1:500|IF/ICC,1:100 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
14KDa/16KDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
LC3B polyclonal antibody detects endogenous levels of LC3B protein.
Applications Notes
Western blot analysis of LC3B expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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