CD133 polyclonal antibody
Product Specifications
Background
CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Prominin-1; Antigen AC133; Prominin-like protein 1; CD133; PROM1; PROML1; MSTP061
Swiss Prot
O43490
Reactivity
Human
Immunogen
Recombinant protein of human CD133.
Conjugation
Unconjugated
Applications
WB IF
Dilution
WB: 1:1000~1:2000 IF: 1:100~1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CD133 polyclonal antibody detects endogenous levels of CD133 protein.
Applications Notes
Western blot (WB) analysis of CD133 polyclonal antibody at 1:1000 dilution Lane1:L02 whole cell lysate (30ug) Lane2:HepG2 whole cell lysate (30ug) Lane3:Panc1 whole cell lysate (30ug) Lane4:H1792 whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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