Smad3 (phospho-S425) polyclonal antibody

Product Specifications

Background

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Swiss Prot

P84022

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 425.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, hippo Signaling, TGFβ Smad signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 56 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Smad3 (S425) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Ser425.

Applications Notes

Western blot (WB) analysis of Smad3 (phospho-Ser425) polyclonal antibody at 1:500 dilution Lane1:A549 treated with λ-phosphatase whole cell lysate (30ug) Lane2:A549 whole cell lysate (30ug) Lane3:A549 treated with TGF-β (10 ng/ml,30 minutes) whole cell lysate (30ug) Lane4:A549 whole cell lysate (30ug)