Smad3 (phospho-S425) polyclonal antibody
Product Specifications
Background
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
Swiss Prot
P84022
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 425.
Conjugation
Unconjugated
Related Pathways
Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, hippo Signaling, TGFβ Smad signaling
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 56 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Smad3 (S425) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Ser425.
Applications Notes
Western blot (WB) analysis of Smad3 (phospho-Ser425) polyclonal antibody at 1:500 dilution Lane1:A549 treated with λ-phosphatase whole cell lysate (30ug) Lane2:A549 whole cell lysate (30ug) Lane3:A549 treated with TGF-β (10 ng/ml,30 minutes) whole cell lysate (30ug) Lane4:A549 whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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