GSK3β (phospho-S9) polyclonal antibody

Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer’s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer’s disease.

Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3B; GSK3beta isoform; Serine/threonine-protein kinase GSK3B

PI3K AKT signaling, Hedgehog Signaling, Hypoxia Signaling, Translational Contral Regulation of elF2, Regulation of Microtubule Dynamics, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, MTOR signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of GSK3β (phospho-Ser9) pAb at 1:500 dilution Lane1:PMVEC whole cell lysate (30ug) Lane2:HepG2 whole cell lysate (30ug) Lane3:HEK293T treated with Calyculin A (50 nM,30 minutes) whole cell lysate (30ug) Lane4:HEK293T whole cell lysate (30ug)