LEF-1 (F38) polyclonal antibody

Product Specifications

Background

Transcription factor that binds DNA in a sequence-specific manner.Participates in the Wnt signaling pathway (By similarity) .Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity) .PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity) .TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1.Regulates T-cell receptor alpha enhancer function.Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity) .May play a role in hair cell differentiation and follicle morphogenesis (By similarity) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha

Swiss Prot

Q9UJU2

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human LEF-1.

Conjugation

Unconjugated

Related Pathways

Adherens Junction signaling, Wntβ catenin signaling

Applications

WB IF

Dilution

WB: 1:1000~1:2000 IF: 1:100~1:500

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 52 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

LEF-1 polyclonal antibody detects endogenous levels of LEF-1 protein.

Applications Notes

Western blot (WB) analysis of LEF-1 polyclonal antibody at 1:1000 dilution Lane1:The brain tissue lysate of Mouse (30ug) Lane2:The brain tissue lysate of Rat (30ug) Lane3:HuT78 whole cell lysate (30ug) Lane4:myla2059 whole cell lysate (30ug)