Catenin-β (Q27) polyclonal antibody
Product Specifications
Background
β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Catenin β; Cateninβ; Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; ORF Names: OK/SW-cl.35; PRO2286
Swiss Prot
P35222
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human Catenin-β.
Conjugation
Unconjugated
Applications
WB IHC IF IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 92 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Catenin-β (Q27) polyclonal antibody detects endogenous levels of Catenin-β protein.
Applications Notes
Western blot (WB) analysis of Catenin-β (Q27) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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