Nephrin (H892) polyclonal antibody
Product Specifications
Background
Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1; NPHN
Swiss Prot
O60500
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 861-910 of Human Nephrin.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:100~1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 135 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Nephrin (H892) polyclonal antibody detects endogenous levels of Nephrin protein.
Applications Notes
Western blot (WB) analysis of Nephrin (H892) polyclonal antibody at 1:1000 dilution Lane1:The muscle tissue lysate of Rat (47ug) Lane2:HEK293T whole cell lysate (41ug) Lane3:Jurkat whole cell lysate (41ug) Lane4:H9C2 whole cell lysate (60ug)
Host or Source
Rabbit
Available Sizes
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