Smad4 (L43) polyclonal antibody

Product Specifications

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4

Swiss Prot

Q13485

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human Smad4.

Conjugation

Unconjugated

Related Pathways

Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, hippo Signaling

Applications

WB IF

Dilution

WB: 1:10000~1:20000 IF: 1:100~1:500

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 75 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Smad4 (L43) polyclonal antibody detects endogenous levels of Smad4 protein.

Applications Notes

Western blot (WB) analysis of Smad4 (L43) polyclonal antibody at 1:10000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:THP-1 whole cell lysate (40ug) Lane6:HepG2 whole cell lysate (40ug)