AKT (A444) polyclonal antibody
Product Specifications
Background
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ
Swiss Prot
P31749/P31751/Q9Y243
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 420-470 of Human AKT1.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Immune Cell Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Regulation of Microtubule Dynamics, T cell receptor signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling
Applications
WB IHC IP IF
Dilution
WB 1:500 - 1:1000 IHC 1:50 - 1:100 IP 1:10 - 1:100 IF: 1:100~1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
AKT (A444) polyclonal antibody detects endogenous levels of total AKT protein.
Applications Notes
Western blot (WB) analysis of AKT (A444) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:The Lung tissue lysate of Rat (40ug) Lane3:The Lung tissue lysate of Mouse (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
