MITF (G174) polyclonal antibody
Product Specifications
Background
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR) . Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) . Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32
Swiss Prot
O75030
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic peptide, corresponding to Human MITF.
Conjugation
Unconjugated
Related Pathways
Wntβ catenin signaling
Applications
WB IF
Dilution
WB: 1:500~1:2000 IF: 1:100~1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 60-75 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MITF polyclonal antibody detects endogenous levels of MITF protein.
Applications Notes
Western blot (WB) analysis of MITF polyclonal antibody at 1:1000 dilution Lane1:The heart tissue lysate of Rat (30ug) Lane2:The kidney tissue lysate of Mouse (30ug) Lane3:CT-26 whole cell lysate (30ug) Lane4:PC12 whole cell lysate (30ug) Lane5:A2780 whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
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